Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines.

Journal: Journal of extracellular vesicles

Volume: 7

Issue: 1

Year of Publication: 

Affiliated Institutions:  Institut Curie, INSERM U, PSL Research University, Paris, France. The Johns Hopkins University School of Medicine, Department of Molecular and Comparative Pathobiology, Baltimore, MD, USA. Brigham and Women's Hospital, Center for Interdisciplinary Cardiovascular Sciences, Boston, MA, USA. Interuniversity Research Institute for Molecular Recognition and Technological Development (IDM), University of Valencia, Polytechnic University of Valencia, Valencia, Spain. University of California, Davis, Department of Otolaryngology, Davis, CA, USA. INSERM U, Université d'Angers, CHU d'Angers, Angers, France. German Centre for Neurodegenerative Diseases (DZNE), Bonn, Germany. Université de Lille, INSERM, U-, Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse - PRISM, Lille, France. University of Lyon, INRA, EPHE, UMR Viral Infections and Comparative Pathology, Lyon, France. La Trobe University, La Trobe Institute for Molecular Science, Department of Biochemistry and Genetics, Bundoora, Australia. Atlantic Cancer Research Institute, Moncton, Canada. Université Bretagne Loire, Oniris, INRA, IECM, Nantes, France. University of Cologne, Department of Internal Medicine I, Cologne, Germany. Royan Institute for Stem Cell Biology and Technology, ACECR, Cell Science Research Center, Department of Stem Cells and Developmental Biology, Tehran, Iran. Massachusetts General Hospital, Department of Neurosurgery, Boston, MA, USA. University of Malta, Department of Pathology, Msida, Malta. University of South Alabama, Department of Pharmacology, Center for Lung Biology, Mobile, AL, USA. University of Technology Sydney, Discipline of Pharmacy, Graduate School of Health, Sydney, Australia. University of Rochester, Rochester, NY, USA. National Institute of Chemistry, Department of Molecular Biology and Nanobiotechnology, Ljubljana, Slovenia. Université Laval, Centre de Recherche du CHU de Québec, Department of Infectious Diseases and Immunity, Quebec City, Canada. Ospedale Santo Spirito, Pescara, Italy. CSGI - Research Center for Colloids and Nanoscience, Florence, Italy. University of Birmingham, Institute of Microbiology and Infection, Birmingham, UK. University of Auckland, Auckland, New Zealand. Jagiellonian University, Faculty of Biochemistry, Biophysics and Biotechnology, Department of Cell Biology, Kraków, Poland. FEMTO-ST Institute, UBFC, CNRS, ENSMM, UTBM, Besançon, France. Institute of Biomedicine and Molecular Immunology (IBIM), National Research Council (CNR) of Italy, Palermo, Italy. Germans Trias i Pujol Research Institute (IGTP), Can Ruti Campus, REMAR-IVECAT Group, Badalona, Spain. INSERM UMR-S , Paris Cardiovascular Research Center, Paris, France. Massachusetts General Hospital and Neuroscience Program, Harvard Medical School, Department of Neurology and Radiology, Boston, MA, USA. Icahn School of Medicine at Mount Sinai, New York City, NY, USA. Harvard University, School of Engineering and Applied Sciences, Cambridge, MA, USA. Nationwide Children's Hospital, Columbus, OH, USA. UMR-CBMN, CNRS-Université de Bordeaux, Bordeaux, France. Haaglanden Medical Center, Department of Neurosurgery, The Hague, The Netherlands. Memorial Sloan Kettering Cancer Center, Department of Medicine, New York City, NY, USA. Lund University, Department of Cardiology, Lund, Sweden. University of Nebraska Medical Center, Department of Pharmacology and Experimental Neuroscience, Omaha, NE, USA. University of Edinburgh, Institute of Immunology & Infection Research, Edinburgh, UK. Kidney Research Centre, Ottawa, Canada. Mayo Clinic, Department of Transplantation, Jacksonville, FL, USA. Technical University of Munich, TUM School of Life Sciences Weihenstephan, Division of Animal Physiology and Immunology, Freising, Germany. University of Torino, Department of Molecular Biotechnology and Health Sciences, Torino, Italy. MTA-SE Immuno-Proteogenomics Research Groups, Budapest, Hungary. University of Michigan, Department of Medicine, Ann Arbor, MI, USA. University of Torino, Department of Medical Sciences, Torino, Italy. Oxford Brookes University, Department of Biological and Medical Sciences, Oxford, UK. University of Auckland, Department of Obstetrics and Gynaecology, Auckland, New Zealand. National Taiwan University Hospital, Department of Internal Medicine, Taipei, Taiwan. National Tsing Hua University, Department of Power Mechanical Engineering, Hsinchu, Taiwan. Leibniz Institute for Farm Animal Biology (FBN), Institute of Reproductive Biology, Dummerstorf, Germany. Cedars-Sinai Medical Center, Los Angeles, CA, USA. Cardiff University, School of Medicine, Cardiff, UK. UNICAMP, Institute of Biology, Campinas, Brazil. The Ohio State University, College of Pharmacy, Division of Pharmaceutics and Pharmaceutical Chemistry, Columbus, OH, USA. Vanderbilt University Medical Center, Epithelial Biology Center, Department of Medicine, Nashville, TN, USA. University of Porto, Faculty of Pharmacy (FFUP), IBMC/IS, Porto, Portugal. University of Oxford, Radcliffe Department of Medicine, Acute Stroke Programme - Investigative Medicine, Oxford, UK. Academic Medical Centre of the University of Amsterdam, Department of Clinical Chemistry and Vesicle Observation Centre, Amsterdam, The Netherlands. The University of Nottingham, School of Medicine, Children's Brain Tumour Research Centre, Nottingham, UK. University of Gothenburg, Institute of Medicine at Sahlgrenska Academy, Krefting Research Centre, Gothenburg, Sweden. University of São Paulo, Ribeirão Preto Medical School, Ribeirão Preto, Brazil. University of Notre Dame, Department of Biological Sciences, Notre Dame, IN, USA. Massachusetts General Hospital, Boston, MA, USA. The Johns Hopkins University School of Medicine, Department of Neurology, Baltimore, MD, USA. IRCCS MultiMedica, Milan, Italy. The Sociedade Beneficente Israelita Brasileira Albert Einstein, São Paulo, Brazil. Cancer Research Institute Ghent, Ghent, Belgium. Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain. Université Catholique de Louvain, Institut de Recherche Expérimentale et Clinique (IREC), Laboratory of Pediatric Hepatology and Cell Therapy, Brussels, Belgium. Universiteit Hasselt, Diepenbeek, Belgium. Aston University, School of Life & Health Sciences, Birmingham, UK. ETH Zurich, Institute of Pharmaceutical Sciences, Zurich, Switzerland. University of L'Aquila, Department of Life, Health and Environmental Sciences, L'Aquila, Italy. Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Buenos Aires, Argentina. TPM of Mirandola, Mirandola, Italy. University of Campinas, Piracicaba Dental School, Department of Oral Diagnosis, Piracicaba, Brazil. Utrecht University, Faculty of Veterinary Medicine, Department of Biochemistry and Cell Biology, Utrecht, The Netherlands. Exogenus Therapeutics, Cantanhede, Portugal. McGill University, Division of Experimental Medicine, Montreal, Canada. James Cook University, Australian Institute of Tropical Health and Medicine, Centre for Biodiscovery and Molecular Development of Therapeutics, Cairns, Australia. University of Gothenburg, Institute of Clinical Sciences at Sahlgrenska Academy, Department of Biomaterials, Gothenburg, Sweden. Evox Therapeutics Limited, Oxford, UK. University of Virginia Health System, Department of Medicine, Division of Nephrology, Charlottesville, VA, USA. CIC bioGUNE, CIBERehd, Exosomes Laboratory & Metabolomics Platform, Derio, Spain. University of São Paulo, Ribeirão Preto Medical School, Department of Pathology and Forensic Medicine, Ribeirão Preto, Brazil. Toronto General Hospital Research Institute, University Health Network, Toronto, Canada. University of Colorado, School of Medicine, Department of Ophthalmology, Cell Sight-Ocular Stem Cell and Regeneration Program, Aurora, CO, USA. Semmelweis University, Department of Genetics, Cell- and Immunobiology, Budapest, Hungary. German Cancer Research Center (DKFZ), Clinical Cooperation Unit Applied Tumor Biology, Heidelberg, Germany. Helmholtz-Centre for Infection Research, Braunschweig, Germany. Karolinska Institute, Department of Medicine Solna, Division for Immunology and Allergy, Stockholm, Sweden. University College London, London, UK. Helmholtz Center Munich German Research Center for Environmental Health, Research Unit Gene Vectors, Munich, Germany. INSERM U, Strasbourg, France. POSTECH (Pohang University of Science and Technology), Department of Life Sciences, Pohang, South Korea. University Hospital Essen, University Duisburg-Essen, Institute for Transfusion Medicine, Essen, Germany. Paracelsus Medical University, GMP Unit, Salzburg, Austria. University of Oxford, Department of Physiology, Anatomy and Genetics, Oxford, UK. BC Cancer, Canada's Michael Smith Genome Sciences Centre, Vancouver, Canada. University Medical Center Göttingen, Developmental Biochemistry, Göttingen, Germany. IRCCS Fondazione Don Carlo Gnocchi, Laboratory of Nanomedicine and Clinical Biophotonics (LABION), Milan, Italy. Loyola University Chicago, Department of Urology, Maywood, IL, USA. University of Toronto, Department of Laboratory Medicine and Pathobiology, Toronto, Canada. Aalborg University Hospital, Department of Clinical Biochemistry, Aalborg, Denmark. University of Massachusetts Medical School, RNA Therapeutics Institute, Worcester, MA, USA. University of Birmingham, Birmingham, UK. Scintillon Institute, La Jolla, CA, USA. Aberystwyth University, Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth, United Kingdom. Imperial College London, London, UK. University Clinic Eppendorf, Hamburg, Germany. Hasselt University, Biomedical Research Institute (BIOMED), Department of Medicine and Life Sciences, Hasselt, Belgium. Nanfang Hospital, Southern Medical University, Department of Clinical Laboratory Medicine, Guangzhou, China. Fondazione IRCCS Istituto Nazionale dei Tumori, Unit of Immunotherapy of Human Tumors, Milan, Italy. The University of Sheffield, Sheffield, UK. Cedars-Sinai Medical Center, Smidt Heart Institute, Los Angeles, CA, USA. Boston University School of Medicine, Boston, MA, USA. University of Hertfordshire, School of Life and Medical Sciences, Biosciences Research Group, Hatfield, UK. Istanbul University Oncology Institute, Basic Oncology Department, Istanbul, Turkey. German Cancer Research Center (DKFZ), Division Signaling and Functional Genomics, Heidelberg, Germany. Copenhagen Lupus and Vasculitis Clinic, Section - Rigshospitalet, Copenhagen, Denmark. University of Maryland, Fischell Department of Bioengineering, College Park, MD, USA. Mayo Clinic, College of Medicine, Department of Physiology and Biomedical Engineering, Rochester, MN, USA. Department of Urology, Rotterdam, The Netherlands. University of Manchester, Division of Cancer Sciences, Manchester Cancer Research Centre, Manchester, UK. National Institutes of Health, National Cancer Institute, Center for Cancer Research, Bethesda, MD, USA. Children's Hospital of Los Angeles, Los Angeles, CA, USA. City of Hope Comprehensive Cancer Center, Beckman Research Institute, Department of Molecular Medicine, Duarte, CA, USA. German Research Center for Environmental Health, Institute for Virology, Munich, Germany. University of Texas MD Anderson Cancer Center, Department of Cancer Biology, Metastasis Research Center, Houston, TX, USA. The Johns Hopkins University School of Medicine, Department of Psychiatry and Behavioral Sciences, Baltimore, MD, USA. National Institutes of Health, National Cancer Institute, Center for Cancer Research, Laboratory of Pathology, Bethesda, MD, USA. National Cancer Center Research Institute, Tokyo, Japan. University of Michigan, Biointerfaces Institute, Ann Arbor, MI, USA. École normale supérieure, Paris, France. Oslo University Hospital, Department of Medical Biochemistry, Blood Cell Research Group, Oslo, Norway. Kyung Hee University, Department of Applied Chemistry, Yongin, Korea. Princess Margaret Cancer Centre, University Health Network, Toronto, Canada. Duke University, Department of Ophthalmology, Durham, NC, USA. West Virginia University, Department of Chemical and Biomedical Engineering and WVU Cancer Institute, Morgantown, WV, USA. German Armed Forces Central Hospital, Department of General, Visceral and Thoracic Surgery, Koblenz, Germany. University of Belgrade, Institute for the Application of Nuclear Energy, INEP, Belgrade, Serbia. University of Mainz, Institute of Developmental Biology and Neurobiology, Mainz, Germany. University Medical Center Hamburg-Eppendorf, Institute of Neuropathology, Hamburg, Germany. Hudson Institute of Medical Research, Melbourne, Australia. Central Research Laboratories, Sysmex Co., Kobe, Japan. Hasselt University, Biomedical Research Institute (BIOMED), Hasselt, Belgium. Finnish Red Cross Blood Service, Research and Development, Helsinki, Finland. Cincinnati Cancer Center, Cincinnati, OH, USA. Thomas Jefferson University, Sidney Kimmel Medical School, Department of Cancer Biology, Philadelphia, PA, USA. University of Virginia, Flow Cytometry Core, School of Medicine, Charlottesville, VA, USA. University of California, San Diego, Department of Obstetrics, Gynecology, and Reproductive Sciences, La Jolla, CA, USA. AstraZeneca, Discovery Sciences, IMED Biotech Unit, Gothenburg, Sweden. Eulji University, School of Medicine, Daejeon, South Korea. Genome Institute of Singapore, ASTAR, Singapore. Federal University of Paraná, Department of Genetics, Human Molecular Genetics Laboratory, Curitiba, Brazil. University of Ljubljana, Faculty of Medicine, Institute of Biochemistry, Ljubljana, Slovenia. University of California, San Diego, Department of Pediatrics, San Diego, CA, USA. University of British Columbia Okanagan, Kelowna, Canada. University of Cambridge School of Clinical Medicine, Addenbrooke's Hospital, Department of Medicine, Cambridge NIHR BRC Cell Phenotyping Hub, Cambridge, UK. Semmelweis University, Department of Physiology, Budapest, Hungary. Institute of Medical Biology (IMB), Agency for Science and Technology (ASTAR), Singapore. Latvian Biomedical Research and Study Centre, Riga, Latvia. Oslo University Hospital-The Norwegian Radium Hospital, Institute for Cancer Research, Department of Molecular Cell Biology, Oslo, Norway. Utrecht University, University Medical Center Utrecht, Center for Molecular Medicine & Regenerative Medicine Center, Utrecht, The Netherlands. Stellenbosch University, Department of Physiological Sciences, Stellenbosch, South Africa. Trinity College Dublin, School of Pharmacy and Pharmaceutical Sciences, Panoz Institute & Trinity Biomedical Sciences Institute, Dublin, Ireland. Harvard University, Harvard T.H. Chan School of Public Health, Boston, MA, USA. Mossakowski Medical Research Centre, NeuroRepair Department, Warsaw, Poland. K.G. Jebsen Brain Tumor Research Centre, Department of Biomedicine, University of Bergen, Bergen, Norway. Utrecht University, University Medical Center Utrecht, Department of Neurosurgery, Brain Center Rudolf Magnus, Institute of Neurosciences, Utrecht, The Netherlands. Mayo Clinic, Rochester, MN, USA. Universitat de València, Departament de Farmàcia i Tecnologia Farmacèutica i Parasitologia, Àrea de Parasitologia, Valencia, Spain. Università degli Studi di Milano, Department of Clinical Sciences and Community Health, EPIGET LAB, Milan, Italy. A.C.Camargo Cancer Center, São Paulo, Brazil. University of Gothenburg, Sahlgrenska Academy, Department of Rheumatology and Inflammation Research, Gothenburg, Sweden. University of Southern California, Los Angeles, CA, USA. SickKids Hospital, Department of Anesthesia and Pain Medicine, Toronto, Canada. Florida State University College of Medicine, Department of Biomedical Sciences, Tallahassee, FL, USA. The School of Biomedical Sciences, University of Western Australia, Perth, Australia. University of Antwerp, Centre for Proteomics, Antwerp, Belgium. Georg-Speyer-Haus Institute for Tumor Biology and Experimental Therapy, Frankfurt, Germany. QIMR Berghofer Medical Research Institute, Herston, Australia. Aalborg University Hospital, Department of Clinical Immunology, Aalborg, Denmark. Erasmus MC, Rotterdam, The Netherlands. Namur Thrombosis and Hemostasis Center (NTHC), NARILIS, Namur, Belgium. University of Padova, Department of Women's and Children's Health, Padova, Italy. Brown University, Women and Infants Hospital, Providence, RI, USA. German Cancer Consortium (DKTK), Partner Site Freiburg and German Cancer Research Center (DKFZ), Heidelberg, Germany. Aarhus University, Department of Clinical Medicine, Aarhus, Denmark. Sorbonne Université, Centre National de la Recherche Scientifique, Research Unit Biology of Adaptation and Aging (BA), Team Compensation in Neurodegenerative and Aging (Brain-C), Paris, France. University of Pisa, Centro Dipartimentale di Biologia Cellulare Cardio-Respiratoria, Pisa, Italy. Universidade Federal do Rio de Janeiro, Instituto de Microbiologia, Rio de Janeiro, Brazil. National Institutes of Health, National Institute on Aging, Baltimore, MD, USA. University of Liège, GIGA-R(MBD), PSI Laboratory, Liège, Belgium. Queen Mary University of London, Blizard Institute, Epigenetics & Cellular Senescence Group, London, UK. National Cancer Center Research Institute, Division of Molecular and Cellular Medicine, Tokyo, Japan. McGill University, The Research Institute of the McGill University Health Centre, Montreal, Canada. IIS-Fundacion Jimenez Diaz-UAM, Department of Nephrology and Hypertension, Madrid, Spain. Graduate School of Public Health at the University of Pittsburgh, Division of Occupational and Environmental Medicine, Pittsburgh, PA, USA. AstraZeneca, Discovery Sciences, IMED Biotech Unit, Cambridge, UK. Statens Serum Institut, Department of Autoimmunology and Biomarkers, Copenhagen, Denmark. University of Buenos Aires, Instituto de Investigaciones Biomédicas en Retrovirus y SIDA (INBIRS), Buenos Aires, Argentina. Amsterdam University Medical Centers, Department of Pathology, Amsterdam, The Netherlands. Spanish National Cancer Research Center (CNIO), Molecular Oncology Programme, Microenvironment and Metastasis Laboratory, Madrid, Spain. IRCCS - Istituto Ortopedico Rizzoli, Laboratory for Orthopaedic Pathophysiology and Regenerative Medicine, Bologna, Italy. The Scripps Research Institute-Scripps Florida, Department of Molecular Medicine, Jupiter, FL, USA. Radboud University Medical Center, Department of Rheumatology, Nijmegen, The Netherlands. Duke University Medical Center, Departments of Medicine and Immunology, Durham, NC, USA. Philipps University Marburg, Experimental Tumor Biology, Marburg, Germany. Pontificia Universidad Católica de Chile, Advanced Center for Chronic Diseases (ACCDiS), Santiago, Chile. CNR Institute of Neuroscience, Milan, Italy. University of California, San Francisco, CA, USA. The Warren Alpert Medical School of Brown University, Department of Medicine, Providence, RI, USA. Department of Veterans Affairs, San Francisco, CA, USA. University of Palermo, Department of Biopathology and Medical Biotechnologies, Palermo, Italy. McGill University, Montreal, Canada. Instituto Oswaldo Cruz, Rio de Janeiro, Brazil. Institut Curie, CNRS UMR, PSL Research University, Paris, France. University of Michigan Medical School, Ann Arbor, MI, USA. Weizmann Institute of Science, Department of Biomolecular Sciences, Rehovot, Israel. University Medical Center Hamburg-Eppendorf, Department of Neurosurgery, Hamburg, Germany. University of Minnesota Medical School, Institute on the Biology of Aging and Metabolism, Department of Biochemistry, Molecular Biology and Biophysics, Minneapolis, MN, USA. CNC, Coimbra, Portugal. Paracelsus Medical University, Department of Transfusion Medicine, Salzburg, Austria. University of Lyon, Lyon-Sud Faculty of Medicine, CarMeN Laboratory (UMR INSERM -INRA ), Pierre-Bénite, France. Maastricht University, GROW, School for Oncology and Developmental Biology, Maastricht Radiation Oncology (MaastRO) Lab, Maastricht, The Netherlands. Sapienza University of Rome, Department of Experimental Medicine, Rome, Italy. West Virginia University, Morgantown, WV, USA. American Red Cross, Scientific Affairs, Gaithersburg, MD, USA. Icahn School of Medicine at Mount Sinai, Department of Medicine, Cardiology, New York City, NY, USA. Advanced Center for Chronic Diseases, Santiago, Chile. Clínica las Condes, Extracellular Vesicles in Personalized Medicine Group, Santiago, Chile. Oregon Health & Science University, Department of Anesthesiology & Perioperative Medicine, Portland, OR, USA. Technische Universität Darmstadt, Department of Biology, Darmstadt, Germany. University Medical Center Utrecht, Laboratory for Clinical Chemistry & Hematology, Utrecht, The Netherlands. University of Bristol, Bristol, UK. University of California, Los Angeles, California NanoSystems Institute, Los Angeles, CA, USA. University of Gothenburg, Institute of Clinical Sciences, Department of Surgery, Sahlgrenska Cancer Center, Gothenburg, Sweden. Research Service, Olin E. Teague Veterans' Medical Center, Temple, TX, USA. Cancer Institute of JFCR, Tokyo, Japan. University of Helsinki, EV Core Facility, Helsinki, Finland. INEB - Instituto de Engenharia Biomédica, Porto, Portugal. Maria Sklodowska-Curie Institute - Oncology Center, Gliwice Branch, Gliwice, Poland. Kansas State University, College of Veterinary Medicine, Manhattan, KS, USA. René Rachou Institute/FIOCRUZ, Belo Horizonte, Brazil. Washington University, Saint Louis, MO, USA. Harvard Medical School, Department of Medicine, Boston, MA, USA. FAU Erlangen-Nuremberg, Transfusion and Haemostaseology Department, Erlangen, Germany. University of Auckland, Department of Molecular Medicine and Pathology, Auckland, New Zealand. Hiroshima University, Institute of Biomedical & Health Sciences, Department of Cellular and Molecular Biology, Hiroshima, Japan. University of Manchester, Manchester, UK. Georgetown University, Department of Medicine, Washington, DC, USA. National University of Singapore, Faculty of Dentistry, Singapore. INSERM U, Aix Marseille University, CNRS UMR, Marseille, France. UNIFESP, Departamento de Ciências Farmacêuticas, Diadema, Brazil. Institut Pasteur de Montevideo, Functional Genomics Unit, Montevideo, Uruguay. Beth Israel Deaconess Medical Center, Boston, MA, USA. University of Perugia, Department of Chemistry, Biology and Biotechnology, Perugia, Italy. University Medical Center Utrecht, Department of Nephrology and Hypertension, Utrecht, The Netherlands. TGen, Neurogenomics Division, Phoenix, AZ, USA. Center for Psychiatry and Neuroscience, INSERM U, Paris, France. Department of Pathology, Erasmus MC, Erasmus Optical Imaging Centre, Rotterdam, The Netherlands. Mayo Clinic, Department of Orthopedic Surgery, Rochester, MN, USA. IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal. University of Kentucky, College of Medicine, Department of Physiology, Lexington, KY, USA. Universidade Federal do Rio Grande do Sul, Instituto de Ciências Básicas da Saúde, Departamento de Microbiologia, Imunologia e Parasitologia, Porto Alegre, Brazil. The Florey Institute of Neuroscience and Mental Health, Melbourne, Australia. UMR CNRS-Université de Lorraine, Vandœuvre-lès-Nancy, France. Oslo University Hospital Rikshospitalet, Research Institute of Internal Medicine, Oslo, Norway. University of Gothenburg, The Sahlgrenska Academy, Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry, Mölndal, Sweden. Case Western Reserve University, Department of Medicine, Cleveland, OH, USA. Vanderbilt University School of Medicine, Department of Cell and Developmental Biology, Nashville, TN, USA. Danube University Krems, Department for Biomedical Research and Christian Doppler Laboratory for Innovative Therapy Approaches in Sepsis, Krems an der Donau, Austria. University of Würzburg, Rudolf Virchow Center, Würzburg, Germany. The University of Vermont Medical Center, Department of Medicine, Burlington, VT, USA. University Hospital RWTH Aachen, Department of Thoracic and Cardiovascular Surgery, Aachen, Germany. Karolinska Institute, Department of Medicine and Center for Molecular Medicine, Respiratory Medicine Unit, Stockholm, Sweden. Chinese Academy of Sciences, Wenzhou Institute of Biomaterials and Engineering, Wenzhou, China. George Papanicolaou Hospital, Public Cord Blood Bank, Department of Haematology - BMT Unit, Thessaloniki, Greece. Universidade Federal de São Paulo Campus Diadema, Departamento de Ciências Farmacêuticas, Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários, São Paulo, Brazil. Xiamen University, Department of Chemical Biology, Xiamen, China. Centro de Biología Molecular Severo Ochoa, Instituto de Investigación Sanitaria la Princesa (IIS-IP), Madrid, Spain. Tsinghua University, School of Pharmaceutical Sciences, Beijing, China. Technical University Eindhoven, Faculty Biomedical Technology, Eindhoven, The Netherlands. University of Padova, Department of Comparative Biomedicine and Food Science, Padova, Italy. Institute of Physiology CAS, Department of Biomaterials and Tissue Engineering, BIOCEV, Vestec, Czech Republic. Vilnius University, Institute of Biomedical Sciences, Department of Physiology, Biochemistry, Microbiology and Laboratory Medicine, Vilnius, Lithuania. Guangzhou Medical University, School of Pharmaceutical Sciences & the Fifth Affiliated Hospital, Key Laboratory of Molecular Target & Clinical Pharmacology, Guangzhou, China. Karolinska Institute, Clinical Research Center, Unit for Molecular Cell and Gene Therapy Science, Stockholm, Sweden. Aix-Marseille Université, Institut Paoli-Calmettes, INSERM U, CNRS UMR, Centre de Recherche en Cancérologie de Marseille, Marseille, France. University of California, Davis, Department of Nutrition, Davis, CA, USA. Exosomics Siena SpA, Siena, Italy.

Abstract summary 

The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.

Authors & Co-authors:  Théry Witwer Aikawa Alcaraz Anderson Andriantsitohaina Antoniou Arab Archer Atkin-Smith Ayre Bach Bachurski Baharvand Balaj Baldacchino Bauer Baxter Bebawy Beckham Bedina Zavec Benmoussa Berardi Bergese Bielska Blenkiron Bobis-Wozowicz Boilard Boireau Bongiovanni Borràs Bosch Boulanger Breakefield Breglio Brennan Brigstock Brisson Broekman Bromberg Bryl-Górecka Buch Buck Burger Busatto Buschmann Bussolati Buzás Byrd Camussi Carter Caruso Chamley Chang Chen Chen Cheng Chin Clayton Clerici Cocks Cocucci Coffey Cordeiro-da-Silva Couch Coumans Coyle Crescitelli Criado D'Souza-Schorey Das Datta Chaudhuri de Candia De Santana De Wever Del Portillo Demaret Deville Devitt Dhondt Di Vizio Dieterich Dolo Dominguez Rubio Dominici Dourado Driedonks Duarte Duncan Eichenberger Ekström El Andaloussi Elie-Caille Erdbrügger Falcón-Pérez Fatima Fish Flores-Bellver Försönits Frelet-Barrand Fricke Fuhrmann Gabrielsson Gámez-Valero Gardiner Gärtner Gaudin Gho Giebel Gilbert Gimona Giusti Goberdhan Görgens Gorski Greening Gross Gualerzi Gupta Gustafson Handberg Haraszti Harrison Hegyesi Hendrix Hill Hochberg Hoffmann Holder Holthofer Hosseinkhani Hu Huang Huber Hunt Ibrahim Ikezu Inal Isin Ivanova Jackson Jacobsen Jay Jayachandran Jenster Jiang Johnson Jones Jong Jovanovic-Talisman Jung Kalluri Kano Kaur Kawamura Keller Khamari Khomyakova Khvorova Kierulf Kim Kislinger Klingeborn Klinke Kornek Kosanović Kovács Krämer-Albers Krasemann Krause Kurochkin Kusuma Kuypers Laitinen Langevin Languino Lannigan Lässer Laurent Lavieu Lázaro-Ibáñez Le Lay Lee Lee Lemos Lenassi Leszczynska Li Liao Libregts Ligeti Lim Lim Linē Linnemannstöns Llorente Lombard Lorenowicz Lörincz Lötvall Lovett Lowry Loyer Lu Lukomska Lunavat Maas Malhi Marcilla Mariani Mariscal Martens-Uzunova Martin-Jaular Martinez Martins Mathieu Mathivanan Maugeri McGinnis McVey Meckes Meehan Mertens Minciacchi Möller Møller Jørgensen Morales-Kastresana Morhayim Mullier Muraca Musante Mussack Muth Myburgh Najrana Nawaz Nazarenko Nejsum Neri Neri Nieuwland Nimrichter Nolan Nolte-'t Hoen Noren Hooten O'Driscoll O'Grady O'Loghlen Ochiya Olivier Ortiz Ortiz Osteikoetxea Østergaard Ostrowski Park Pegtel Peinado Perut Pfaffl Phinney Pieters Pink Pisetsky Pogge von Strandmann Polakovicova Poon Powell Prada Pulliam Quesenberry Radeghieri Raffai Raimondo Rak Ramirez Raposo Rayyan Regev-Rudzki Ricklefs Robbins Roberts Rodrigues Rohde Rome Rouschop Rughetti Russell Saá Sahoo Salas-Huenuleo Sánchez Saugstad Saul Schiffelers Schneider Schøyen Scott Shahaj Sharma Shatnyeva Shekari Shelke Shetty Shiba Siljander Silva Skowronek Snyder Soares Sódar Soekmadji Sotillo Stahl Stoorvogel Stott Strasser Swift Tahara Tewari Timms Tiwari Tixeira Tkach Toh Tomasini Torrecilhas Tosar Toxavidis Urbanelli Vader van Balkom van der Grein Van Deun van Herwijnen Van Keuren-Jensen van Niel van Royen van Wijnen Vasconcelos Vechetti Veit Vella Velot Verweij Vestad Viñas Visnovitz Vukman Wahlgren Watson Wauben Weaver Webber Weber Wehman Weiss Welsh Wendt Wheelock Wiener Witte Wolfram Xagorari Xander Xu Yan Yáñez-Mó Yin Yuana Zappulli Zarubova Žėkas Zhang Zhao Zheng Zheutlin Zickler Zimmermann Zivkovic Zocco Zuba-Surma

Study Outcome 

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Statistics
Citations :  References, especially those provided to illustrate methods and approaches, are representative only, and are not meant to be a comprehensive review of the literature. Most references were derived from suggestions provided in the MISEV2018 Survey results. Each reference was checked by multiple authors. Citation implies deemed relevance of scientific content and not an endorsement by the authors or ISEV of any particular journal or editorial practice.
Authors :  382
Identifiers
Doi : 1535750
SSN : 2001-3078
Study Population
Male,Female
Mesh Terms
Other Terms
ectosomes;exosomes;extracellular vesicles;guidelines;microparticles;microvesicles;minimal information requirements;reproducibility;rigor;standardization
Study Design
Study Approach
Country of Study
Publication Country
United States